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Literature summary extracted from

  • Shipman, L.W.; Li, D.; Roessner, C.A.; Scott, A.I.; Sacchettini, J.C.
    Crystal structure of precorrin-8x methyl mutase (2001), Structure, 9, 587-596.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.4.99.61 expression in Escherichia coli Pseudomonas denitrificans (nom. rej.)

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.4.99.61 apo form and complexed with hydrogenobirinate, hanging drop vapor diffusion with equal volumes of concentrated protein, i.e. 10 mg/ml, and a precipitant solution containing 25%-32.5% polyethylene glycol 4000, 200 mM MgCl2, 100 mM Tris-HCl, pH 8.5, crystals diffract to 2.1 A and 2.6 A resolution Pseudomonas denitrificans (nom. rej.)

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.4.99.61 Hydrogenobyrinate
-
 Pseudomonas denitrificans (nom. rej.)

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.4.99.61 0.00091
-
 Hydrogenobyrinate
-
 Pseudomonas denitrificans (nom. rej.)

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.4.99.61 22060
-
 gel filtration, native-PAGE Pseudomonas denitrificans (nom. rej.)

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.4.99.61 Precorrin-8X Pseudomonas denitrificans (nom. rej.) involved in the aerobic pathway to vitamin B12 Hydrogenobyrinate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.4.99.61 Pseudomonas denitrificans (nom. rej.) P21638
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.4.99.61 recombinant CoBH Pseudomonas denitrificans (nom. rej.)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.99.61 Precorrin-8X
-
 Pseudomonas denitrificans (nom. rej.) Hydrogenobyrinate
-
 ?
5.4.99.61 Precorrin-8X involved in the aerobic pathway to vitamin B12 Pseudomonas denitrificans (nom. rej.) Hydrogenobyrinate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.4.99.61 CoBH
-
 Pseudomonas denitrificans (nom. rej.)

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
5.4.99.61 0.00017
-
 Hydrogenobyrinate product inhibition Pseudomonas denitrificans (nom. rej.)